Vimentin Antibody Cell Signaling

The antibody is expected to exhibit the same species cross reactivity as the unconjugated vimentin d21h3 xp rabbit mab 5741.

Vimentin antibody cell signaling.

Western blot analysis of extracts from control hela cells lane 1 or vimentin knockout hela cells lane 2 using vimentin r28 antibody upper or β actin 13e5 rabbit mab 4970 lower. The hrp conjugated anti rabbit igg antibody gtx213110 01 was used to detect the primary antibody. The absence of signal in the vimentin knockout hela cells confirms specificity of the antibody for vimentin. An antibody shouldn t be one of the variables in your experiment.

The absence of signal in the vimentin knockout hela cells confirms specificity of the antibody for vimentin. The vimentin antibody sampler kit provides an economical means to detect total levels of vimentin vimentin phosphorylated at ser56 and vimentin phosphorylated at ser82. Vimentin s dynamic structural changes and spatial re organization in response to extracellular stimuli help to coordinate various signaling pathways 4. Various whole cell extracts 30 μg were separated by 10 sds page and the membranes were blotted with vimentin antibody gtx100619 diluted at 1 5000 and competitor s antibody diluted at 1 5000.

Western blot analysis of extracts from various cell lines using vimentin. Vimentin s dynamic structural changes and spatial re organization in response to extracellular stimuli helps to coordinate various signaling pathways 13. Find out why customers rank cst highest for antibody specificity and sensitivity. Rabbit mab 4970 lower.

The kit contains enough primary and secondary antibody to perform two western blot experiments. This cell signaling technology antibody is conjugated to alexa fluor 647 fluorescent dye and tested in house for direct flow cytometry and immunofluorescent analysis in human cells. β catenin is a key downstream effector in the wnt signaling pathway 14. Visit the supplier page for more detailed antibody product information such as specificity and validated applications.

Vimentin is an intermediate filament of mesenchymal origin and is present at early developmental stages. Monoclonal antibody for studying vimentin in the cytoskeletal signaling research area. The absence of signal in the vimentin knockout hela cells confirms specificity of the antibody for vimentin.