T Cell Activation Kit

Cloudz human t cell activation kit is optimized for t cell expansion when used in combination with excellerate human t cell expansion media xeno free and cytokines including il 2 il 7 and il 15.

T cell activation kit.

Primary human cd3 cells were activated with gmp cloudz human t cell activation kit and cultured for 9 days in excellerate t cell expansion media r d systems catalog ccm030 and 20 ng ml il 2 r d systems catalog 202 gmp. The kit consists of anti biotin macsibead particles and biotinylated antibodies against human cd2 cd3 and cd28. For some studies cells must be treated with biological response modifiers. Anti biotin macsibead particles loaded with the biotinylated antibodies are used to mimic antigen presenting cells and activate resting t cells from pbmcs as well as purified t cells.

The nuclear factor of activated t cells nfat il 2 or other kinds of factors will be detected by bioluminescent methods or elisa kits. A t cell is a type of lymphocyte which develops in the thymus gland hence the name and plays a central role in the immune response t cells can be distinguished from other lymphocytes by the presence of a t cell receptor on the cell surface these immune cells originate as precursor cells derived from bone marrow and develop into several distinct types of t cells once they have migrated to. Meanwhile the t cell activation and proliferation process can also be evaluated via cell counting kit 8 cck 8 mtt cfse as well as flow cytometric analysis etc. Ique t cell companion kits.

The activation and cytokine growth factor combinations used with this kit should be optimized by application or experimental protocol. The t cell activation expansion kit was developed for the activation and expansion of mouse t cells. The ique t cell companion kits can be used to measure additional cytokines besides those already included in the ique t cell activation cell and cytokine profiling kit or other t cell immunology kits. Cell counts were performed to determine percent viability on days 5 7 and 9 of the experiment compared to.

Induction of apoptosis one of the most common ways to assess t cell activation is to measure t cell proliferation upon in vitro stimulation of t cells via antigen or agonistic antibodies to tcr. Below is the list of available cytokines for t cell companion kits. We guarantee to provide the best class. The t cell activation expansion kit was developed to activate and expand human t cells.

Dynabeads human t activator cd3 cd28 offer a simple method for activation and expansion of t cells that does not require feeder cells antigen presenting cells or antigen. The uniform 4 5 µm diameter inert superparamagnetic beads are similar in size to antigen presenting cells and are covalently coupled to anti cd3 and anti cd28 antibodies. Induction of cytotoxicity or cytokine secretion 5. The bd phosflow t cell activation kit catalog no.

560750 provides the reagents necessary to simultaneously analyze multiple t cell markers with one of several intracellular phosphoproteins for the purpose of studying t cell subpopulation specific signaling events. Differentiation into effector cells 4.