Sted Live Cell Imaging

However the diffraction limited resolution of light microscopy is often inadequate forcing researchers to complement the live cell imaging strategy by em.

Sted live cell imaging.

Live cell imaging of dendritic spines by sted microscopy. Often the growth of cells cell aggregates or cell movement is observed over time using compound microscopes and contrasting methods like phase contrast and differential interference contrast dic also time lapse imaging of larger specimens e g. Short and long term effects of live cell sted imaging on cos7 and hela cells. As sted microscopy is an extremely powerful tool to visualize extremely small structures we hope to apply these types of photoresistant molecules to study biological events such as the formation of actin filaments in cells says taki.

Valentin nägerl a 1 2 katrin i. Live cell imaging is a microscopy technique that allows in vivo imaging of cells instantly and over a period of time. By using two color sted with multi lifetime imaging three channel sted is possible. Willig b 1 birka hein b stefan w.

Time lapse fluorescence imaging has become one of the most important approaches in neurobiological research. Lifetime imaging and advanced time gating. In particular both confocal and two photon microscopy have been used to study activity dependent changes in synaptic morphology. Developing zebrafish embryos is widely performed.

Live cell images were taken by yoshikatsu sato the chief coordinator at the live imaging center of itbm. Live cell imaging of dendritic spines by sted microscopy u. There are different types of microscopy compatible with live cell imaging which include both conventional contrast techniques like differential interference contrast dic or phase contrast and fluorescence based techniques. Adaptive illumination for superresolution sted imaging at minimal light dosages.

18982 18987 view in article. The range of microscopic techniques applied for live cell imaging is also extremely wide. Multicolor sted has also been used to show that different populations of synaptic vesicle proteins do not mix of escape synaptic boutons. Early on sted was thought to be a useful technique for working with living cells.

Hell b 3 and tobias bonhoeffer a 3 a department of cellular and systems neurobiology max planck institute of neurobiology 82152 martinsried germany.