Stably Expressing Cell Line

Most cell biology experiments utilize transient transfection protocols that afford peak gene expression between 24 96 hours post transfection.

Stably expressing cell line.

Altogen labs provides a stable cell line development service by transforming your cell line to stably express a vector or gene of interest including rnai and tet inducible cell lines. Generation of stably expressing cell lines in 28 days. Company scientists bring years of. Egfr wild type stably expressing ba f3 cell line.

Generation of monoclonal cell lines from expansion of individual a549 cells stably expressing cas9. This is done through the cloning of plasmid dna that encodes your gene of interest a correct promoter. We offer generation of stable cell lines by transforming your cell line or one of our 120 in house cell lines and primary cells to stably express vector or gene of interest. Once you have obtained resistant cell lines you should expand the cells and assay your target gene compared with positive and negative control.

Stable cell lines over expressing a protein of interest or shrna construct targeting a gene of interest are important research tools particularly for the production of recombinant antibodies and other proteins the. Altogen labs has unique experience with more than 150 cancer cell lines customizable reporter systems highly efficient gene delivery technologies as well as the research expertise to produce stably expressing clonal cells in just 28 days read more about generation of stable cell lines in 28 days service. You can detect the expression of fusion protein by an appropriate analysis method such as western blot microscopy elisa as well as flow cytometry. A549 cells were transduced with lenticas9 blast and then selected with 1 µg ml blasticidin for 9 days.

However if sustained gene expression is required for longer periods of time generation of stable cell lines is a viable option. A stable cell line was developed following puromycin antibiotic selection. This cell line along with its matching fluorescent ligand can be used in a tag lite receptor binding application. Generation of stably expressing cell lines in 28 days altogen labs can provide development of stable cell lines in 28 days.

Plasmid dna construct should encode at least one antibiotic resistance gene to enable drug selection. Generation of stably expressing cell lines in 28 days. In the current study human neuroblastoma sh sy5y cell lines that stably express wt and mt egfp tagged frmd7 were enginerred using a lentiviral approach. Epidermal growth factor receptor egfr is a cell surface receptor with intrinsic intracellular protein tyrosine kinase tk activity.

Analysis of stable cell lines. Ligand binding induces egfr dimerization and phosphorylation leading to the activation of egfr signaling pathway.